Molecular Biology

Molecular Biology Section was founded in 1991 by Prof. dr. Tülin AKTAÇ. It is composed of 1 professor, 1 associate professor, 1 assistant professor and, 2 research associates. Molecular Biology, Biochemistry I and II, Biochemistry Laboratory, Histology, Histology Laboratory, Endocrinology, Cell Biology Laboratory (required courses) and Antibiotics, Molecular Changes in Cancer Cells, Vitamins, Cell Communication Mechanisms, and Special Topics on Cell Biology (selective courses) are the contributions of Molecular Biology section to the undergraduate education. Molecular Biology section also contributes to graduate education by the courses of Biochemical Methods, Industrial Enzymes, Enzymatic Regulations, Biosensors, Human Biochemistry, Genome Organization and Regulation, Membrane Physiology, Fungal Enzymes, Techniques in Electron Microscopy, Cell Damage and Adaptation, Cell Signals, Oxidant Stress and Antioxidant Defense Mechanisms.

Since the foundation of section, 10 Ph.D. thesis, 24 M.Sc. thesis have been supervised by the academic staff of the section. In addition, academic staff have assigned in 38 BAP and 2 TUBITAK projects as principal investigators and researchers.

The research topics studied by the academic staff members are characterization and purification of enzymes and proteins, bio production and isolation of industrial enzymes like amylase, inulinase and protease, immobilization of enzymes by varying techniques, investigation of different applications by immobilized enzymes, determination of antioxidant and antimicrobial potential of natural compounds, the assessment of beta-glucan existence in fungi and its potential use in diabetes, investigation of the effects of some antioxidants and stem cell applications on ischemia reperfusion damage in gut, heavy metal toxicity in plants, apoptotic effects of some bacteria on cancer cell lines, toxicity of food additives, antioxidant and healing effects of proantocyanidine and fucoidane, varying oxidative stress parameters and effects of xenobiotics on these parameters, determination of gene expression levels of fungal enzymes and optimization of production conditions effecting gene expression levels in fungi. The studies performed by the members of division have been published in the journals indexed by SCI and SCI-Exp and other indexed journals.

List of indexed journals in which articles were published by academic members of The Molecular Biology Division:

J.Environ. Bio (SCI Exp)

Preparative Biochemistry and Biotechnology ( SCI ve SCI Exp.)

J. Chem. Soc. Pak.(SCI)

Food Technol. Biotechnol. (SCI Exp.)

Biomedicine & Pharmacotherapy (SCI)

Biologia (SCI Exp)

International Journal of Pharmacology (SCI Exp)

Romanian Biotechnological Letters (SCI Exp.)

Biotechnolgy and Biotechnological Equipment( SCI Exp.)

J Pediatr Surg (SCI)

Bull. Environ. Contam. Toxicol. (SCI and SCI Exp)

Acta Physiol. Plant. (SCI Exp)

Biotechnic & Histochemistry(SCI Exp)

Environmental Toxicology(SCI Exp)

Bull. Environ. Contam. Toxicol. (SCI Exp)

J Molecular Histollogy(SCI Exp)

Experimental Molecular Pathology(SCI Exp)

J Med Food(SCI Exp)

Turk J Biol. (SCI Exp)

Fresenius Environ. Bull (SCI Exp)

Andrologia (SCI Exp)

Rev. Chim (SCI Exp)

Food Science and Technology (Diğer Uluslararsı İndexler)

J. of Pharmaceutical and Biological Sciences (Diğer Uluslararsı İndexler)

J.Chem. Soc. Pak. (SCI Exp)

Journal of Applied Biological Sci. (Diğer Uluslararsı İndexler)

Uludağ Üniversitesi Tıp Fakültesi Dergisi (TR Dizin)

Bıomedıcıne  Pharmacotherapy (SCI-Exp)

Biotechnic  Histochemistry (SCI-Exp)

J.BioSci, Biotech (Diğer Uluslararsı İndexler)

Fresenius Environmental Bulletin (SCI-Exp)

International Journal of Pharmacology (SCI-Exp)

Plant and Soil (SCI)

Mikrobiyoloji Bülteni (SCI-Exp)

45 articles in SCI and SCI-Exp. Journals, 15 articles in international indexed journals, 15 abstracts/posters in international congress and 22 abstracts/posters in national congress and 20 articles in nationally indexed journals and 4 book chapters were published by the members of Molecular Biology Division.

List of techniques used in the researches conducted in the division:

-      Separation techniques of enzymes and protein molecules

-      Purification and investigation of molecules by chromatographic techniques

-      Ion-exchange chromatography

-      Gel filtration chromatography

-      Affinity gel chromatography

-      Thin layer chromatography (TLC)

-      Electrophoresis (SDS-PAGE, Native-PAGE)

-      Immobilization methods

-      Spectroscopy methods

-      Fermentation techniques (SSF, SmE)

-      PCR

-      Cell culture

-      Techniques used in cell death determination 

-      Techniques used in apoptosis detection

-      TALI Image-based cell cytometer

-      Gene expression studies of fungal enzymes

-      In-situ localization of some molecules and immunochemical techniques (TUNEL, Bax/Bcl2, eNOS, iNOS)

-      Cytopathological, histopathological and morphologic ultra-structural techniques with TEM

-      Basic and specific cell staining techniques by paraffin method