Molecular Microbiology and Biotechnology

MEMBERS OF OUR RESEARCH GROUP:

Ece ŞEN, Ph.D., Professor of Microbiology

Deniz AKSOY, Ph.D., Teaching Assistant

 Hatice SOYLU, M.Sc., Graduate student

Gamze KAZAR, M.Sc., Graduate student

 Müjdat ÖZGÜR,  M.Sc., Graduate student

 Süleyman DOĞRU, Bio.,Graduate  student

 Büşra ÖZEN, Bio.,Graduate student

OUR REASEARCH AREAS:

Molecular identification of water and food borne pathogens 
Emerging spirochetes from environmental and zoonotic sources

Determinants of pathogenesis of Salmonella sp. bacteria and current  molecular methods  for determination of virulence 
Assesment of carcinogenic materials in water sources and other substances by using biosensors and bacteriological Ames test  
Assesment of emerging water and food borne pathogens , their isolation and genotyping
Utilization of new indicators of microbial water pollution  
Isolation of tick and field mice borne bacterial pathogens, their molecular typing  and genotyping  (Borrelia burgdorferi, Borrelia turcica, Leptospira sp.) 
Biological control and effects of new insecticides 
Molecular determination of bacteria-cancer relationships

Antibiotic resistance mechanisms of Salmonella sp.

Antibiotic resistance profiles of food borne Salmonella sp.

Bioinsecticides and new methods in bioinsecticide production

Assesment of insecticide resistance against Bacillus thuringiensis and bacterial insecticides by using bioassays and molecular genetics methods

 

Resim

Borrelia turcica sp.novo (researchers: E.Şen et al.)

Resim
Salmonella enteritidis Typhimurium (researchers :E.Şen , D.Aksoy, H.Soylu, G.Kazar)

METHODS USED BY OUR RESEARCH GROUP:

Polymerase chain reaction (gradient and real time PCR) 
Agarose gel electrophoresis
 
Molecular probes , primer design
 
DNA/RNA transfer-Blotting techniques
 
Plasmid analysis
 
Genotyping, ribotyping
 
Southern blotting
 
Northern blotting
 
Gene cloning
 
Cell cultures
 
Biological assays (Animal assays)(Balb/c mice, field mice)
 
Biyosensors and Ames mutagenesis test
 

Gradient PCR

Real Time PCR

Molecular and enzyme based methods for determination of apoptosis

TALI visual cell cytometry

Resim
(Hyalomma sp. ;Molecular identification of tick borne pathogens found in our country ) (Researchers: E.Şen, H.Soylu)

AIMS OF OUR RESEARCH GROUP:

Determination of tick species and their distribution sampled in Edirne and surroundings

Isolation and genotyping of tick and mice borne pathogens from Edirne and surroundings 
Isolation and molecular determination of virulence of food and water borne pathogens in our area 
Molecular investigation of bacteria-cancer relationships  
Biological control of ticks and determination of effects of non-toxic novel insecticides on ticks and their hosts  
Development of new molecular methods for identification of pathogen Salmonella sp.  
Assesment of efficiency of newly introduced indicator microorganisms of water pollution 
Investigation of water sources by using bacteriological and mutagenic tests and determination of emerging pathogens in our area 
Teaching students of our department and students from  health-related fields the molecular microbiology techniques and new microbiological methods
Teaching our students to design new biotechnological products such as new diagnostic kits, DNA chips, new microbial insecticides by using the methods of molecular genetics and microbiiology

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